Service Details Service Charges How To Order
All mouse strains imported from not approved commercial suppliers must be re-derived into the TCP animal facility.
Re-derivation involves obtaining embryos from the required strain that are extensively washed and transferred into SPF pseudopregnant recipients. Tissue samples from re-derived offspring are provided to the investigator for genotyping. Re-derived offspring is tested to verify their health status before the release - see health monitoring (foster mothers)
All strains of mice can be re-derived, but the efficiency of the procedure depends on the robustness and fecundity of the strain. Inbred strains may require larger starting numbers. It is possible to eliminate viral (e.g. MHV, Sendai, parvovirus), bacterial (e.g. mycoplasma), and ectoparasite (e.g. pinworm, mites) infections. Using this method we re-derived 140 strains from contaminated mouse stock of SLRI Mount Sinai Hospital in preparation for the move into TCP in the summer of 2007.
Please, refer to Policies and Procedures for the required documentation such as valid Animal Use Protocol and Mouse Passport prior to the initiation of re-derivation.
To maximize the chances of successful re-derivation 3-5 male mice at breeding age are imported into TCP containment. Typically wild type female mice of necessary background are used as embryo donors. If it is absolutely essential to maintain the specific background and/or genotype of the strain, equal or double the number of female mice should be imported as well to initiate breeding colony in containment before proceeding with rederivation.
Following steps are required:
- Submit mouse passport and AUP amendment to add the new mouse strain to TCP LIMS.
- Submit the Live Animal Importation Form from LIMS to Import/Export co-ordinator
- Submit the re-derivation service request
Alternative ways of re-derivation into TCP:
- Shipment of freshly collected pre-implantation stage embryos co-ordinated with TCP TG core for immediate surgery on arrival. This route eliminates the need of live animals' shipment, their housing in containment, purchase of wild type female embryo donors, mating and embryo collection. Contact to co-ordinate the shipment of fresh embryos to TCP.
- Shipment of frozen embryos or frozen sperm to CMMR for temporary storage and cryorecovery. See CMMR Services for further details on fees and service description.
- Cryorecovery of frozen embryos from Samuel Lunenfeld Research Institute Mount Sinai Hospital or CMMR archive of frozen embryos. See CMMR Services for further details on fees and service description
- Mating co-ordination with local animal facilities, delivery of dissected oviducts to TCP
*For re-derivation from animals imported into TCP containment we will use wild type females of necessary background as embryo donors. If it is absolutely necessary to maintain the specific background and/or genotype of the strain and wild type donor females are not a feasible option for embryo collection and re-derivation, equal or double the number of females should be imported together with the males and breeding in containment will have to be initiated before we can proceed with rederivation.
We also can co-ordinate fresh embryo shipment for the export of your animals from TCP for rederivation by the receiving colony.
||Mount Sinai &
|External Canadian Academic
||Other External Academic
| Animals housed in TCP (base fee per re-derivation attempt)
| Dissected oviducts delivered to TCP (base fee per re-derivation attempt)
| Fresh Embryo shipment (base fee per re-derivation attempt)
| Embryo transfer surgery (first recipient)
| (each additional recipient)
|For details and fees on embryo cryorecovery from SLRI/CMMR archive or other repositories please, see CMMR Services.
How to Order
- Refer to Policies and Procedures for the required documentation prior to the initiation of the re-derivation
- PI and the lab contact register at TCP LIMS
- Submit a service request: Login to LIMS
from the top menu go to Service Requests -> Request a TCP Service page
, search with Department set to "TCP Transgenic Core and Specialty Resources", then select the Re-Derivation service request using appropriate source of embryos