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Re-Derivation

Service Details      Service Charges      How To Order


Service Details

All mouse strains imported from not approved commercial suppliers must be re-derived into the TCP animal facility.

Re-derivation involves obtaining embryos from the required strain that are extensively washed and transferred into SPF pseudopregnant recipients. Tissue samples from re-derived offspring are provided to the investigator for genotyping. Re-derived offspring is tested to verify their health status before the release - see health monitoring

All strains of mice can be re-derived, but the efficiency of the procedure depends on the robustness and fecundity of the strain. Inbred strains may require larger starting numbers. It is possible to eliminate viral (e.g. MHV, Sendai, parvovirus), bacterial (e.g. mycoplasma), and ectoparasite (e.g. pinworm, mites) infections. Using this method we re-derived 140 strains from contaminated mouse stock of Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital in preparation for the move into TCP in the summer of 2007.

Please, refer to Policies and Procedures for the required documentation such as valid Animal Use Protocol and Mouse Passport prior to the initiation of re-derivation.

To maximize the chances of successful re-derivation 3-5 male mice at breeding age are imported into TCP containment. Typically wild type female mice of necessary background are used as embryo donors. If it is absolutely essential to maintain the specific background and/or genotype of the strain, equal or double the number of female mice should be imported as well to initiate breeding colony in containment before proceeding with rederivation.

Following steps are required:

  1. Submit mouse passport and AUP amendment to add the new mouse strain to TCP LIMS.
  2. Submit the Live Animal Importation Form from LIMS to Import/Export co-ordinator
  3. Submit the re-derivation service request

Alternative ways of re-derivation into TCP:

  1. Shipment of freshly collected pre-implantation stage embryos co-ordinated with TCP TG core for immediate surgery on arrival. This route eliminates the need of live animals' shipment, their housing in containment, purchase of wild type female embryo donors, mating and embryo collection. Contact to co-ordinate the shipment of fresh embryos to TCP.
  2. Shipment of frozen embryos or frozen sperm to CMMR for temporary storage and cryorecovery. See CMMR Services for further details on fees and service description.
  3. Cryorecovery of frozen embryos from 3. Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital or CMMR archive of frozen embryos. See CMMR Services for further details on fees and service description
  4. Mating co-ordination with local animal facilities, delivery of dissected oviducts to TCP


We also can co-ordinate fresh embryo shipment for the export of your animals from TCP for rederivation by the receiving colony.

Service Charges

Service Mount Sinai &
Sick Kids
Hospitals
External Canadian Academic Other External Academic
Animals housed in TCP (base fee per re-derivation attempt)
$265 $345 $425
Dissected oviducts delivered to TCP (base fee per re-derivation attempt)
$212 $275 $339
Fresh Embryo shipment (base fee per re-derivation attempt)
$159 $207 $255
Embryo transfer surgery (first recipient)
$129 $168 $207
Embryo transfer surgery (each additional recipient)
$97 $127 $156
For details and fees on embryo cryorecovery from please see CMMR Services.
    Service fees include:
  • surgical embryo transfer into SPF pseudopregnant females following
  • alternatively:
    • superovulation, mating and collection of embryos
    • embryo harvest from dissected oviducts delivered to TCP
    • recovery of fresh embryos shipped to TCP Transgenic Core
    Service fees do not include (extra charges apply):
  • purchase of female embryo donors of required strain background for each rederivation attempt (number equal to the number of available embryo donor males)
  • animal housing (per diem) charges for donors in containment
  • animal housing (per diem) charges for recipients and their offspring from the embryo transfer surgery
  • weaning
  • tissue biopsy
  • export fees and shipping charges

How to Order

  1. Refer to Policies and Procedures for the required documentation prior to the initiation of the re-derivation
  2. PI and the lab contact register at TCP LIMS
  3. Submit a service request: Login to LIMS
    from the top menu go to Service Requests -> Request a TCP Service page
    , search with Department set to "TCP Transgenic Core and Specialty Resources", then select the Re-Derivation service request using appropriate source of embryos

 

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