Miscellaneous Mouse Services
Service Details 
Service Charges 
How To Order
Service Details
Timed pregnancy service is provided to the investigators for their experimental studies. To generate timed pregnant mice, females in estrus are selected and mated in holding C on Floor 2, vaginal plugs are checked next morning. Pregnant females are delivered to the investigators holding rooms on Floor 2 or to The Sterile Tissue Harvest Room on the ground floor on the required day of gestation for dissections. Pregnancy can not be guaranteed, the charge is applied based on the number of plugs provided.
Superovulation allows to maximize the number of embryos and to minimize the number of embryo donors. Mouse embryo donor ICR females are hormonally induced to ovulate by intraperitoneal injection of hormones: PMSG (pregnant mare's serum gonadotropin) and hCG (human chorionic gonadotropin). Injected animals are placed with breeder ICR males after hCG injection. Mated animals are identified by the presence of a vaginal copulation plug. Plugged embryo donors are used for the collection of pre-implanttaion mouse embryos between E0.5 and E3.5 and are delivered to The Sterile Tissue Harvest Room on the ground floor on the required day.
Pseudopregnant recipients for embryo transfers are prepared by mating CD-1 females in estrus with individually housed vasectomized CD-1 males in holding A, females are checked for vaginal copulation plugs. Pseudopregnant females at E0.5 day are used for embryo transfer into the oviducts and at E2.5 for the transfer into the uterus. Pseudopregnant recipients are provided to the investigators for their experimental studies and can be delivered to the Surgical Suite on Floor 1 on the required day.
Embryo transfer involves the surgical transfer of embryos into the oviducts or uterus of anesthetized pseudopregnant recipients. Surgeries are performed in the laminar flow hood of the dedicated Surgical Suite.
These services can be requested from the TCP Transgenic Core provided the experimental procedures are described in the Investigator's own Animal Use Protocol
Service Charges
| Service |
Mount Sinai and Sick Kids Hospitals and University of Toronto Faculty of Medicine
|
External Canadian Academic |
Other External Academic |
| Timed pregnant naturally mated ICR female from holding B (per plug) |
$25 |
$33 |
$40 |
Timed pregnant naturally mated C57BL/6 female from holding B (per plug)
|
$40 |
$52 |
$64 |
Superovulated and mated ICR female (per mouse)
|
$25 |
$33 |
$40 |
| Pseudopregnant female
(per mouse) |
$40 |
$52 |
$64 |
Embryo Transfer Surgery including animal cost (per mouse)
|
$90 |
$11 |
$144 |
Superovulation and mating on Floor 1 - NOT including animal cost (per mouse)
|
$10 |
$13 |
$16 |
Superovulation and mating on Floor 2 - NOT including animal cost (per mouse)
|
$15 |
$20 |
$24 |
Collection of preimplantation stage embryos (per plug)
|
$15 |
$20 |
$24 |
Embryo shipment - admin fee (per shipment)
|
$75 |
$98 |
$120 |
|
|
How to Order
- Register for TCP LIMS account
- Login to LIMS and from the top menu go to Service Requests -> Request a TCP Service page
, search with Department set to "TCP Transgenic Core and Specialty Resources", then select the appropriate service request
- Sign up on the Calendar online
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