| ES Cell Services |
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Gene Targeting using validated ES cell parental lines from the Mount Sinai Hospital Samuel Lunenfeld Research Institute ES cell facility |
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Expansion of ES Cell Clones and Their Preparation for the Generation of Chimeras |
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Derivation of Novel ES Cell Lines from mutant mice housed at the TCP animal facility |
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| Generation of Chimeras |
- morula aggregation
- morula/blastocyst microinjection
- tetraploid complementation assay
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| Pronuclear Microinjection |
Transgenic production by pronuclear microinjection of the zygotes |
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| Re-Derivation |
Re-derivation of mouse strains to Specific Pathogen-Free (SPF) status |
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| Miscellaneous Mouse Services |
- Naturally mated and superovulated timed pregnancy for the dissection of different embryonic stages;
- Pseudopregnant recipients for embryo transfers;
- Embryo transfer surgeries.
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