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Policies and Procedures

Required documentation for the animal use

Investigators housing animals at TCP are required to have a TCP Animal Use Protocol (AUP) submitted to TCP's Animal Care Committee Coordinator for review prior to the submission of a Service Request. We cannot initiate any experiments without a valid Animal Use Protocol.

If you already have an approved TCP AUP on file, please, complete mouse passport on TCP LIMS and submit a TCP AUP amendment to add the new mouseline (s) to your existing AUP.

Investigators not planning to house animals in TCP i.e. exporting re-derived or newly generated by TG core animals into their institutions are not required to have a TCP AUP (the procedures involved in rederivation and generation of genetically modified animals for external investigators are covered by the Transgenic Core AUP). However, it is essential to have all necessary paperwork in place in the home institution to effectively transfer the imported animals to their facilities. Please verify the institutional requirements for importing animals prior to arranging the experiments with TCP TG core.

Health Monitoring

All re-derived, cryorecovered and newly generated animals are housed in the TCP core barrier holding area B not accessible to researchers after embryo transfer surgery. As part of health monitoring program in this area, generated animals are screened directly using PCR and pooled fecal samples before being released from Transgenic Core. Samples from foster mothers - embryo recipients are sent to an external commercial diagnostic laboratory for testing. All health monitoring fees are charged back to the investigator's account.

Animals are released after murine pathogen testing has been completed, and results are reviewed and approved by the veterinarian. At this point they can be transferred to other holding areas within TCP including barrier holding A or exported

Pathogen-test of Embryonic Stem (ES) cells

The evidence of murine pathogens screening is part of TCP requirements to test all biological materials prior to their introduction into SPF animals. PCR-based Alternative to MAP (mouse antibody production) testing offered at IDEXX and Charles River Laboratories are used for screening ES cells clones used for generation of chimeras.

TCP Transgenic Core accepts ES cell clones from the following facilities:

  1. Approved Toronto ES cell facilities at Sick Kids Hospital; Samuel Lunenfeld Research Institute at Mount Sinai Hospital; CMMR; TCP Transgenic Core and repositories of International Knockout Mouse Consortium (IKMC). Cell samples from these facilities are regularly tested and results are provided to TG core.
  2. All ES cells originating from or propagated in other laboratories must be tested prior to the initiation of chimeras' generation experiments. TCP Transgenic Core offers a service to grow untested ES cell clones in a quarantine hood and incubator and send the samples for a test before generation of chimeras on a cost-recovery basis

Publications

We ask the investigator to acknowledge the TCP Transgenic Core facility in the scientific publications resulting from our services and provide the copies of these publications to the TCP Transgenic Core.

 

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