TCP Transgenic Core Service Fees

Payment

Submission of a Service Request on LIMS is required prior to the initiation of any service by the TCP Transgenic Core. After the service is performed the invoice is issued on a monthly basis. Cage per diem fees are NOT included in the fees listed for the Transgenic Core services and are charged separately to the investigator's account from the embryo transfer surgery onwards. The fees listed are in Canadian dollars. Please refer to the Service Agreements for more details.

The TCP Transgenic Core Facility is supported in part by Sick Kids Hospital, Samuel Lunenfeld Research Institute at Mount Sinai Hospital and the Faculty of Medicine of University of Toronto.

Transgenic Core Service Agreements

Volume discounts offered from May 1, 2009 to frequent users of the following services:

Generation of chimeras by Aggregation with diploid or tetraploid embryos
OR Blastocyst/ Morula microinjection
Transgenic Production by Pronuclear Microinjection

Terms and Conditions:

This pricing promotion will expire in one year: May 1, 2010.
Experiments #2 - 5 will have 2.5 - 10 % step discounts of the regular prices (see below).
A 10% discount off the regular price is offered for each successive experiment.
Every 6th experiment will receive a special incentive of a 30% discount off the regular price instead of 10% off (applies to experiments # 6, 12, 18, 24, etc).
The experimental categories can be combined (e.g. #1 - Pronuclear microinjection; #2 - Aggregation; #3 - Blastocyst/Morula microinjection).
The experiments will be tracked for the purpose of this pricing promotion.
The discounts will be applied in the month following the charge.

Experiment #	% Discount	Experiment #	% Discount	Experiment #	% Discount
1	Regular price	7	10	13	10
2	2.5	8	10	14	10
3	5	9	10	15	10
4	7.5	10	10	16	10
5	10	11	10	17	10
6	30	12	30	18	30

Fees (as of May 1, 2009)

Service	Mount Sinai and Sick Kids Hospitals and University of Toronto Faculty of Medicine	External Canadian Academic	Other External Academic	For Profit Organizations
ES Cell Services
Gene targeting (per experiment)	$5000	$6500	$8000	$10,000
Expansion of positive clones in addition to five included (per clone)	$100	$130	$160	$200
Expansion of 96 well plate in 24 wells if required (per 24 well plate)	$200	$260	$320	$400
Counting chromosomes (up to 3 clones)	$300	$390	$480	$600
Counting chromosomes (each additional clone)	$150	$195	$240	$300
Preparation of ES cells for outsourced karyotype analysis (per clone)	$100	$130	$160	$200
Expansion of ES cell clones from other facilities (per clone)	$300	$390	$480	$600
Preparation of ES cells for aggregation or microinjection (per clone)	$77	$100	$123	$154
Derivation of novel ES cell lines (base fee per attempt)	$500	$650	$800	$1000
Expansion of derived ES cell lines (per each new line)	$50	$65	$80	$100
Generation of Chimeras
Aggregation with tetraploid OR diploid ICR, EGFP, EYFP, or RFP embryos (per attempt)	$2035	$2646	$3256	$4070
C57BL/6 blastocyst/morula microinjection (per attempt)	$2255	$2932	$3608	$4510
ICR blastocyst/morula microinjection (per attempt)	$2195	$2854	$3512	$4390
Breeding chimeras for germline (per ES cell line) - NOT including animal purchase, cage per diem and tissue biopsies	$200	$260	$320	$400
Mouse identification and tissue biopsies (per cage)	$10	$13	$16	$20
Pronuclear Microinjection
Pronuclear microinjection into ICR zygotes (per attempt)	$2035	$2646	$3256	$4070
Pronuclear microinjection into FVB and B6 x B6D2 zygotes (per attempt)	$2195	$2854	$3512	$4390
Re-Derivation
Animals housed in TCP containment (per re-derivation attempt)	$450	$585	$720	$900
Dissected oviducts delivered to TCP or animals housed in TCP on Floor 2 (per re-derivation attempt)	$400	$520	$640	$800
Embryo shipment or cryorecovery from SLRI archive (per re-derivation attempt)	$350	$455	$560	$700
Miscellaneous Mouse Services
Timed pregnant naturally mated ICR female from holding C on Floor 2 (per plug)	$25	$33	$40	$50
Timed pregnant naturally mated C57BL/6 female from holding C on Floor 2 (per plug)	$35	$46	$56	$70
Superovulated and mated ICR female (per mouse)	$25	$33	$40	$50
Pseudopregnant female (per mouse)	$40	$52	$64	$80
Embryo Transfer Surgery (per mouse)	$50	$65	$80	$100
Superovulation NOT including animal cost (per mouse)	$10	$13	$16	$20
Collection of preimplantation stage embryos (per plug)	$15	$20	$24	$30
Embryo shipment for export - admin fee (per shipment)	$75	$98	$120	$150