TCP Transgenic Core Service Fees
Payment
TCP LIMS account and Submission of a Service Request on TCP LIMS is required prior to the initiation of any service by the TCP Transgenic Core. The invoice is issued on a monthly basis. Additional variable charges not included in the fees below are applied directly to the investigator's account:
- Animal housing - cage per diem from the embryo transfer surgery onwards
- Health monitoring
- Exportation and courier fees as necessary
The TCP Transgenic Core Facility is grateful for generous support provided by Sick Kids Hospital and Samuel Lunenfeld Research Institute at Mount Sinai Hospital.
Transgenic Core Service Agreements
Fees
Fee structure
Effective April 1, 2013 the service fees for the generation of chimeras, transgenic founders and ES cells manipulations increased by 3%. The service fees for timed pregnancies, weaning and tissue biopsies remained the same.
| Service |
Subsidized Internal Rate
(SLRI Mt. Sinai and SickKids hospitals) |
External Canadian Academic |
Other External Academic |
| ES Cell Services |
| Electroporation and isolation of antibiotic-resistant ES cell clones (per attempt - 2 cuvettes/maximum 2x 96 well plates)) |
$1545 |
$2009
|
$2472 |
| Expansion of positive clones and new ES cell lines (per clone) |
$57 |
$74
|
$91 |
| Additional vials or cell pellets (per vial) |
$36 |
$47
|
$58 |
| Expansion of 96 well plate in 24 wells if required (per 24 well plate) |
$206 |
$268 |
$330 |
| Expansion of ES cell clone from other facility (per clone) 15% discount for each additional clone from the same source expanded at the same time |
$309 |
$402 |
$494 |
| ESC preparation for aggregation/microinjection (per clone) - includes refreezing |
$103 |
$134 |
$165 |
| Derivation of novel ES cell lines (base fee per attempt)- does not include expansion and cryopreservation |
$515 |
$670 |
$824 |
| Generation of Chimeras |
| Aggregation with diploid ICR embryos (per attempt) |
$2204 |
$2865 |
$3527 |
Aggregation with tetraploid ICR or EGFP embryos (per attempt)
|
$2369 |
$3080 |
$3790 |
| C57BL/6 morula microinjection (per attempt) |
$2204 |
$2865 |
$3527 |
| ICR morula microinjection (per attempt) |
$2204 |
$2865 |
$3527 |
| Breeding chimeras for germline (per chimera) - does not include cage per diem, animal purchase and tissue biopsies |
$52 |
$68 |
$83 |
| Pronuclear Microinjection |
| Pronuclear microinjection into ICR, FVB or B6x B5D2 zygotes (per attempt) |
$2204 |
$2865 |
$3527 |
| Re-Derivation* |
| BASE fee -Animals housed in TCP (per attempt) |
$258 |
$368 |
$412 |
BASE fee - Dissected oviducts delivered to TCP (per attempt)
|
$206 |
$268 |
$332 |
BASE fee -Fresh Embryo shipment (per attempt)
|
$155 |
$201 |
$247 |
Embryo transfer surgery (first recipient)
|
$126 |
$164 |
$202 |
Embryo transfer surgery (each additional recipient)
|
$95 |
$123 |
$151 |
* The Re-derivation fees do not include animal purchase of embryo donors, cage per diem and tissue biopsies
* For details and fees on embryo cryorecovery from SLRI/CMMR archive or other repositories please, see CMMR Services |
| Miscellaneous Mouse Services |
Timed pregnant naturally mated ICR female from holding B (per plug)
|
$33.70 |
$43.80 |
N/A |
Timed pregnant naturally mated C57BL/6 female from holding B (per plug)
|
$76.50 |
$99.50 |
N/A |
Superovulated and mated ICR female (per mouse)
|
$29 |
$38 |
N/A |
| Pseudopregnant female
(per mouse) |
$44 |
$57 |
N/A |
Superovulation and mating - NOT including animal cost (per mouse)
|
$16 |
$21 |
$26 |
Collection of preimplantation stage embryos (per plug)
|
$16 |
$21 |
$26 |
Embryo shipment - admin fee (per shipment)
|
$77 |
$100 |
$124/td>
|
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