TCP Transgenic Core Service Fees

Payment

Submission of a Service Request on LIMS is required prior to the initiation of any service by the TCP Transgenic Core. After the service is performed the invoice is issued on a monthly basis. Additional variable charges not included in the fees below but applied directly to the investigator's account are:

Animal housing - cage per diem from the embryo transfer surgery onwards
Health monitoring
Exportation and courier fees as necessary

The TCP Transgenic Core Facility is grateful for generous support provided in part by Sick Kids Hospital, Samuel Lunenfeld Research Institute at Mount Sinai Hospital and the Faculty of Medicine of University of Toronto.

Transgenic Core Service Agreements

Fees (Year 2010-2011)

Service	Mount Sinai and Sick Kids Hospitals and University of Toronto Faculty of Medicine	External Canadian Academic	Other External Academic
ES Cell Services
Gene targeting (per experiment)	$5000	$6500	$8000
Expansion of 96 well plate in 24 wells if required (per 24 well plate)	$200	$260	$320
Preparation of ES cells for aggregation or microinjection (per clone) - does NOT include re-freezing	$77	$100	$123
Expansion of ES cell clone from other facility (per clone/line)	$300	$390	$480
ESC expansion, subcloning, preparation for karyotyping, aggregation/microinjection (per clone) - includes refreezing	$100	$130	$160
Derivation of novel ES cell lines (base fee per attempt)- does not include expansion	$500	$650	$800
Expansion of newly derived ES cell lines and subclones (per line/subclone)	$50	$65	$80
Counting chromosomes (up to 3 clones)	$300	$390	$480
Counting chromosomes (each additional clone)	$150	$195	$240
Generation of Chimeras
Aggregation with diploid ICR embryos (per attempt)	$2035	$2646	$3256
Aggregation with tetraploid ICR or EGFP embryos (per attempt)	$2035	$2646	$3256
C57BL/6 blastocyst/morula microinjection (per attempt)	$2255	$2932	$3608
ICR blastocyst/morula microinjection (per attempt)	$2195	$2854	$3512
Breeding chimeras for germline (per chimera) - does not include cage per diem, animal purchase and tissue biopsies	$50	$65	$80
Mouse identification and tissue biopsies (per cage)	$10	$13	$16
Pronuclear Microinjection
Pronuclear microinjection into ICR zygotes (per attempt)	$2035	$2646	$3256
Pronuclear microinjection into FVB zygotes (per attempt)	$2195	$2854	$3512
Pronuclear microinjection into B6 x B6D2 zygotes (per attempt)	$2195	$2854	$3512
Re-Derivation
Animals housed in TCP containment (per re-derivation attempt)	$450	$585	$720
Dissected oviducts delivered to TCP or animals housed in TCP on Floor 2 (per re-derivation attempt)	$400	$520	$640
Fresh Embryo shipment * (per re-derivation attempt)	$350	$455	$560
* For details and fees on embryo cryorecovery from SLRI/CMMR archive or other repositories please, see CMMR Services
Miscellaneous Mouse Services
Timed pregnant naturally mated ICR female from holding B (per plug)	$25	$33	$40
Timed pregnant naturally mated C57BL/6 female from holding B (per plug)	$40	$52	$64
Superovulated and mated ICR female (per mouse)	$25	$33	$40
Pseudopregnant female (per mouse)	$40	$52	$64
Embryo Transfer Surgery including animal cost (per mouse)	$90	$11	$144
Superovulation and mating on Floor 1 - NOT including animal cost (per mouse)	$10	$13	$16
Superovulation and mating on Floor 2 - NOT including animal cost (per mouse)	$15	$20	$24
Collection of preimplantation stage embryos (per plug)	$15	$20	$24
Embryo shipment - admin fee (per shipment)	$75	$98	$120