TCP Transgenic Core Service Fees
Payment
Submission of a Service Request on TCP LIMS is required prior to the initiation of any service by the TCP Transgenic Core. The invoice is issued on a monthly basis. Additional variable charges not included in the fees below but applied directly to the investigator's account are:
- Animal housing - cage per diem from the embryo transfer surgery onwards
- Health monitoring
- Exportation and courier fees as necessary
The TCP Transgenic Core Facility is grateful for generous support provided by Sick Kids Hospital and Samuel Lunenfeld Research Institute at Mount Sinai Hospital.
Transgenic Core Service Agreements
Fees
Fee structure changes and increase
Effective October 1, 2011 the service fees for the generation of transgenic mice on ICR background and aggregation chimeras will increase to reflect the actual cost of the service. At the same time, the service fees for the generation of transgenic mice on B6 x B6D2 and FVB background, as well as injection chimeras will be reduced. All services will continue to be charged per attempt and users are reminded that some experiments may require more than one session. The new pricing will be reflected on service requests scheduled to begin after October 1, 2011.
As recommended by the TG users Committee, re-derivation and ES cell service fees are now itemized to better reflect the workflow and the cost. The service fees for timed pregnancies, weaning and tissue biopsies offered by TG core will be harmonized with similar services of TCP.
The Executive Committee have also approved future increases for the TG core fees to a minimum of 2% annually.
| |
Subsidized Internal Rate
(SLRI Mt. Sinai and SickKids hospitals) |
External Canadian
Academic Rate |
External
Academic Rate |
| Current |
October 1, 2011 |
Current |
October 1, 2011 |
Current |
October 1, 2011 |
| Aggregation with diploid ICR embryos |
$2035 |
$2140 |
$2646 |
$2782 |
$3256
|
$3424 |
| Aggregation with tetraploid ICR embryos |
$2035 |
$2300 |
$2646 |
$2990 |
$3256 |
$3680 |
| Blastocysts/morula microinjection |
$2255 |
$2140 |
$2939 |
$2782 |
$3608
|
$3424 |
Pronuclear microinjection (ICR)
|
$2035 |
$2140 |
$2646 |
$2782 |
$3256 |
$3424 |
| Pronuclear microinjection (FVB and B6 x B6D2) |
$2195 |
$2140 |
$2854 |
$2782 |
$3512
|
$3424 |
| Superovulated & mated ICR female (per plug) |
$25.00 |
$27.50 |
$33.00 |
$35.75 |
N/A |
N/A |
| Timed pregnant naturally mated ICR female (per plug) |
$25.00 |
$33.70 |
$33.00 |
$43.81 |
N/A |
N/A |
| Timed pregnant naturally mated C57BL/6NCrl female (per plug) |
$40.00 |
$76.50 |
$52.00 |
$99.45 |
N/A |
N/A |
| Service |
Mount Sinai &
Sick Kids
Hospitals |
External Canadian Academic |
Other External Academic |
| ES Cell Services |
| Electroporation and isolation of antibiotic-resistant ES cell clones (per attempt) |
$1500 |
$1950
|
$2400 |
| Expansion of positive clones and new ES cell lines (per clone) |
$55 |
$72
|
$88 |
| Additional vials or cell pellets (per vial) |
$35 |
$46
|
$56 |
| Expansion of 96 well plate in 24 wells if required (per 24 well plate) |
$200 |
$260 |
$320 |
| ESC preparation for aggregation/microinjection (per clone) - does NOT include re-freezing |
$77 |
$100 |
$123 |
| Expansion of ES cell clone from other facility (per clone/line) |
$300 |
$390 |
$480 |
| ESC preparation for aggregation/microinjection (per clone) - includes refreezing |
$100 |
$130 |
$160 |
| Derivation of novel ES cell lines (base fee per attempt)- does not include expansion |
$500 |
$650 |
$800 |
| Generation of Chimeras |
| Aggregation with diploid ICR embryos (per attempt) |
$2035 |
$2646 |
$3256 |
Aggregation with tetraploid ICR or EGFP embryos (per attempt)
|
$2035 |
$2646 |
$3256 |
| C57BL/6 blastocyst/morula microinjection (per attempt) |
$2255 |
$2932 |
$3608 |
| ICR blastocyst/morula microinjection (per attempt) |
$2195 |
$2854 |
$3512 |
| Breeding chimeras for germline (per chimera) - does not include cage per diem, animal purchase and tissue biopsies |
$50 |
$65 |
$80 |
| Pronuclear Microinjection |
| Pronuclear microinjection into ICR zygotes (per attempt) |
$2035 |
$2646 |
$3256 |
Pronuclear microinjection into FVB zygotes (per attempt)
|
$2195 |
$2854 |
$3512 |
Pronuclear microinjection into B6 x B6D2 zygotes (per attempt)
|
$2195 |
$2854 |
$3512 |
| Re-Derivation |
| BASE fee -Animals housed in TCP (per attempt) |
$250 |
$325 |
$400 |
BASE fee - Dissected oviducts delivered to TCP (per attempt)
|
$200 |
$260 |
$320 |
BASE fee -Fresh Embryo shipment (per attempt)
|
$150 |
$195 |
$240 |
Embryo transfer surgery (first recipient)
|
$120 |
$156 |
$192 |
Embryo transfer surgery (each additional recipient)
|
$90 |
$117 |
$144 |
* The fees do not include animal purchase of embryo donors, cage per diem and tissue biopsies
* For details and fees on embryo cryorecovery from SLRI/CMMR archive or other repositories please, see CMMR Services |
| Miscellaneous Mouse Services |
Timed pregnant naturally mated ICR female from holding B (per plug)
|
$25 |
$33 |
$40 |
Timed pregnant naturally mated C57BL/6 female from holding B (per plug)
|
$40 |
$52 |
$64 |
Superovulated and mated ICR female (per mouse)
|
$25 |
$33 |
$40 |
| Pseudopregnant female
(per mouse) |
$40 |
$52 |
$64 |
Superovulation and mating - NOT including animal cost (per mouse)
|
$15 |
$20 |
$24 |
Collection of preimplantation stage embryos (per plug)
|
$15 |
$20 |
$24 |
Embryo shipment - admin fee (per shipment)
|
$75 |
$98 |
$120 |
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