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Sperm Services

Service Details

Service Charges

How To Order


Service Details

Sperm Cryopreservation

Sperm Cryopreservation is used to preserve male germ cells of a given mouseline. Fresh sperm is harvested, assessed for sperm number, morphology and motility, and cryopreserved from males provided by the Requester.

Each session of Sperm Cryopreservation includes harvest, assessment, washing and cryopreservation of sperm in 12-14 frozen aliquots. Each aliquot consists of sperm suspended in a cryoprotectant compound, frozen in straws, and stored in bulk liquid nitrogen storage tanks at two separate locations to ensure safety of the archive. For best results, proven male breeders, 8 to 35 weeks old, that have been housed apart from females for two to three days should be used. The Requester should ensure that they have at least two proven male breeders remaining in their colony to continue live breeding until Quality Control experiments are complete. While Sperm Cryopreservation is less expensive than embryo cryopreservation, recovery of the mouseline can be more expensive. For each mouseline cryopreserved with sperm, one session of Quality Control, either in vitro or to live born for all sperm cryopreservation requests is necessary to ensure the quality of the frozen archive.

Choose Sperm Cryopreservation when you want to preserve a mouseline with male germ cells and can recover the line using wild-type donor oocytes. At least one generation of subsequent breeding will be required to generate homozygous mice.

Service fees are per male and include sperm harvest, assessment, and cryopreservation.

Service fees do not include sperm storage fees, if applicable, Quality Control, or animal import and housing charges for male donors, if applicable.

In Vitro Sperm Quality Control

In vitro Sperm Quality Control is used to test frozen-thawed sperm for fertility and ability to generate blastocysts after sperm cryopreservation. A frozen aliquot of sperm is thawed, assessed for sperm concentration, morphology and progressive motility, and used for IVF followed by embryo culture. This is the minimum necessary quality control that must accompany all Sperm Cryopreservation requests.

Each session of In vitro Sperm Quality Control includes thawing and assessment of sperm followed by IVF with oocytes collected from 5 CD-1/ICR donor females. Strain matched donor females can be used for an additional charge. Strain-matched oocyte donors may give a better indication of sperm fertility given strain differences in the ability of sperm to penetrate the oocyte zona pellucida of different strains. After IVF, embryos are collected and cultured for 24 hours to the 2-cell stage and counted. Embryos are then cultured for another 48-72 hours (up to 96 hours total) to blastocysts and counted. These numbers are reported back to the investigator and give an indication of sperm quality following cryopreservation and thawing.

Service fees are per sperm sample and include post-thaw sperm assessment, CD-1 oocyte donors, oocyte harvest, IVF and quantification of 2-cell embryos after 24 h and blastocysts after 96 h culture.

Service fees do not include Sperm Cryopreservation, oocyte donors from other strain backgrounds, embryo collection, Embryo Cryopreservation or Quality Control to Live Born.

Sperm Quality Control to Live Born

Sperm Quality Control to Live Born is used to test frozen-thawed sperm for fertility and ability to generate live born mice after sperm cryopreservation. A frozen aliquot of sperm is thawed, assessed for sperm concentration, morphology and progressive motility, and used for IVF followed by embryo transfer. This is recommended to accompany Sperm Cryopreservation requests when Sperm Cryopreservation is used as a method of preserving mouse lines that are to be discontinued.

Each session of Sperm Quality Control to Live Born includes thawing and assessment of sperm followed by IVF with oocytes collected from 5 CD-1/ICR donor females. Strain matched donor females can be used for an additional charge equal to the difference in purchase cost. Strain-matched oocyte donors may give a better indication of sperm fertility given strain differences in the ability of sperm to penetrate the oocyte zona pellucida of different strains. After IVF, embryos are transferred to CD-1 pseudopregnant recipients and allowed to develop to term. The pups are counted and tissue biopsies are collected for submission to requester for genotyping. These numbers are reported back to the investigator and give an indication of sperm quality following cryopreservation and thawing.

Choose Sperm Quality Control to Live Born when you want to test in vitro fertilization ability of cryopreserved sperm to generate live born offspring. This is especially recommended when Sperm Cryopreservation is used as a method of preserving mouse lines that are to be discontinued.

Service fees are per sperm sample and include post-thaw sperm assessment, washing, CD-1 oocyte donors, oocyte harvest, IVF, embryo transfer of up to 100 embryos, pseudopregnant recipients, and collection of tissue biopsies from live born offspring.

Service fees do not include Sperm Cryopreservation, oocyte donors from other mouse strains, or animal housing.


Sperm Cryorecovery

Sperm cryorecovery is used to re-establish a mouseline from cryopreserved sperm. Frozen sperm is thawed and used for in vitro fertilization (IVF) with wild type oocytes to generate embryos. One straw of sperm is usually used to fertilize the oocytes from 10-20 superovulated females. The resultant embryos are assessed for in vitro viability and transferred into pseudopregnant females. Pregnant females and litters are housed in the SPF barrier at the TCP until pups are shipped to the Requester at his/her expense. If permitted by the Requester's home animal facility, weaned pups can be shipped directly to the user, without health testing.

From CMMR Stock

The Principal Investigator (PI) or designate must complete the following steps to request Sperm Cryorecovery from CMMR stock:

If resultant mice are to be shipped across international borders, appropriate animal export/import regulations must be followed. All necessary paperwork for shipping and receipt of animals is the responsibility of the PI.

The PI or designate must complete a Service Request form on TCP LIMS entitled Cryo Recovery from Sperm before the CMMR will schedule the service request. All CMMR stocks have existing TCP LIMS Mouseline Passports to enable service request entry.

The CMMR will thaw the sperm, perform IVF and embryo assessment. The resulting embryos will be transferred into pseudopregnant recipients. The PI and designate will be able to access his/her request form on TCP LIMS to see the progress of his/her request.

Service fees are per straw of sperm and include the recovery of sperm from CMMR stock, thawing the sperm, superovulation of oocyte donors, oocyte harvest, IVF and embryo assessment and washing.

Service fees do not include purchase of commercial oocyte donors, embryo transfer, pseudopregnant recipients and their housing, clipping or genotyping of pups, health testing of foster mothers, animal housing after weaning, or shipping costs. Embryo transfer, pseudopregnant recipients, and animal housing are not included in the cost since these costs will vary depending on the number of embryos produced and transferred. In general, each recipient will receive approximately 20 embryos and will be housed for ~43 days until litters are weaned.

From FIMRe or Other Repositories

Cryopreserved sperm from other repositories can also be recovered at the CMMR. This is helpful when sperm or mice are located outside of Canada as shipping and import of germ cells is often easier than that of live mice. Several steps must be completed by the Principal Investigator (PI) or designate:

Complete and have approved all paperwork associated with the importation of sperm into Canada, including any Material Transfer Agreements, CMMR Sperm Import Form, customs, and animal tissue import forms required. The PI should forward copies of all forms required to accompany the shipment to the originating repository. All necessary paperwork for shipping and receipt of animals or sperm is the responsibility of the PI.

The PI or designate should place and pay for the order of sperm. Once all paperwork and financial items have been arranged, the PI or designate should put the supplying repository in touch DIRECTLY with CMMR staff (lauryl.nutter@phenogenomics.ca) so that we can arrange shipping of the frozen sperm from the supplying repository straight into the CMMR. All shipping is at the PI's expense unless included in the costs paid to the supplying repository. In any case, a courier account number should be provided to the CMMR for use for any shipping expenses not covered. These include the cost of return shipping of any dry shippers used to transport frozen sperm.

The PI or designate must complete a Service Request form on TCP LIMS entitled Cryo Recovery from Sperm before the CMMR will schedule shipping of the sperm. For strains new to the TCP LIMS, this will require that the PI or designate complete a Mouseline Passport for the mouseline prior to completing the service request form. Please see R ülicke et al. (2007) Laboratory Animals41:301-311 for guidelines for nomenclature of genetically-engineered mice.

The CMMR will generate embryos by IVF and transfer embryos to recover the mouseline. The PI and designate will be able to access his/her request form on TCP LIMS to see the progress of his/her request.

Service fees are per straw of sperm and include communications between the CMMR and supplying repository, the receipt of sperm from the supplying repository, superovulation of oocyte donors, thawing the sperm, oocyte harvest, IVF, and embryo assessment.

Service fees do not include charges from supplying repository, purchase of commercial oocyte donors, embryo transfer, pseudopregnant recipients and their housing, clipping or genotyping of pups, health testing of foster mothers, animal housing or shipping costs. Embryo transfer, pseudopregnant recipients and animal housing are not included in the cost since these costs will vary depending on the number of embryos produced and transferred. In general, each recipient will receive approximately 20 embryos and will be housed for ~43 days until litters are weaned.

Sperm Straw Withdrawal

Shipping of cryopreserved germ cells and embryos, especially across international boundaries, is often simpler than shipping live mice. As a result, investigators who have the local ability to recover live mice from cryopreserved embryos or germ cells can purchase cryopreserved stock from our publicly available mouselines.

Service fees are per straw and include the recovery and packaging of cryopreserved mouselines from CMMR stock.

Service fees do not include shipping, which is done via the courier and account number provided by the investigator at the time of their request.

Sperm Storage

Sperm samples are divided and stored at two different sites in storage vessels under 24-hr surveillance to ensure safety of the frozen archive.

Service Charges

Service SickKids TCP Investigators Non-Profit Canadian Institution Non-Profit International Institution
Sperm Cryopreservation
Sperm Cryopreservation, per male
  • Requester provides carrier/mutant male breeder(s)
  • Includes sperm harvest, assessment, washing and cryopreservation
  • Does not include animal transport or housing, quality control in IVF, or sperm storage fees, if applicable

TCP F1 or F2

$250

$325

$325

$400

Containment or External

$300

$390

$390

$480

In vitro Sperm Quality Control, per sperm sample
  • Assessment of frozen-thawed sperm for in vitro fertilization rates to blastocyst stage
  • Includes post-thaw sperm assessment, CD-1 oocyte donors, oocyte harvest, IVF and quantization of 2-cell embryos after 24 h and blastocysts after 96 h culture
  • Does not include sperm cryopreservation or oocyte donors from other mouse backgrounds or QC to live born

$310

$377

$400

$491

Sperm Quality Control to Live Born, per sperm sample
  • Assessment of frozen-thawed sperm for production of live born mice
  • Includes post-thaw sperm assessment, washing, CD-1 oocyte donors, oocyte harvest, IVF and quantization of 2-cell embryos after 24 h, embryo transfer surgery & recipients for up to 60 embryos, and tail biopsy of live born pups.
  • Does not include sperm cryopreservation, oocyte donors from other mouse strains, or animal housing

$550

$660

$715

$880

Annual Storage Charge, per mouseline
  • Storage of up to 30 straws of sperm at two different sites in monitored liquid nitrogen storage tanks

$30

$39

$39

$48

Sperm Cryorecovery
Sperm Cryorecovery, per straw from CMMR stock
  • Includes recovery of frozen sperm from CMMR stock, sperm thaw and assessment, oocyte harvest, IVF and embryo assessment and washing
  • Does not include material cost of sperm straw, if applicable, purchase and superovulation of oocyte donors, embryo transfer and recipients, or animal housing or shipping costs

$545

$675

$705

$870

Sperm Cryorecovery, per straw from other repository
  • Includes communication with supplying repository, receipt and temporary storage of sperm, sperm thaw and assessment, oocyte harvest, IVF and embryo assessment and washing
  • Does not include charges from supplying repository, shipping, purchase and superovulation of oocyte donors, embryo transfer surgery and recipients, or animal housing costs

$680

$850

$880

$1085

Embryo Transfer, per recipient
  • Includes embryo transfer of ~20 embryos per pseudopregnant recipient
  • Total costs per Service Request depends on total number of embryos transferred
  • Does not include animal housing, health testing of individual recipients, clipping or genotyping of pups, or animal transport

$95

$95

$124

$152

Sperm Straw, per straw from CMMR stock
  • Cost of sperm straw from available CMMR stock
  • Does not include sample export administration fees, if applicable

$240

$315

$315

$384

Use of Dry Shipper, per shipment
  • Includes use of CMMR dry shipper for the transport of sperm or embryos
  • Does not include charging with liquid nitrogen or shipping costs

$75

$97.50

$97.50

$120

Charging Dry Shipper, per use
  • Includes labour and liquid nitrogen to charge dry shipper for the transport of embryos or sperm
  • Does not include use of dry shipper or shipping costs

$30

$39

$39

$48


How to Order

The Principal Investigator (PI) (and designate) must have an account on TCP LIMS. Please follow the Order Instructions with the relevant Order Form below.

Service Service Request Form
Sperm Cryopreservation
Sperm Cryopreservation Sperm Cryopreservation
Sperm Cryorecovery
Sperm Cryorecovery Cryo Recovery from Sperm
Sperm Straw Germ Cell and Embryo Withdrawal

 

 

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