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Research & Support  

The Centre for Modeling Human Disease (CMHD)

Under the direction of Dr. Janet Rossant, a world leader in mouse developmental genetics, the CMHD provides core facilities for generation and analysis of mutant mice to the local and national community and participates in international initiatives in large-scale mutagenesis of the mouse genome. The CMHD consists of six specialized cores:

  1. Mutagenesis Core,
  2. Physiology Core,
  3. Learning, Memory, and Neurophysiology Core,
  4. Pathology Core,
  5. Gene Trap Core, and
  6. Mouse Genetic Mapping Core

By combining the power of mouse mutagenesis with a barrage of phenotypic screens that address areas such as diabetes, hematology, bone mineralization, cardiovascular and renal function, learning, memory and behaviour, and embryonic development, the CMHD is an invaluable resource for researchers worldwide.

The CMHD utilizes complementary, comprehensive approaches to high-throughput, genome-wide functional analysis – chemical mutagenesis of mice by ethylnitrosourea (ENU), and insertional mutagenesis of mouse embryonic stem (ES) cells. ENU generates random point mutations into spermatogonial stem cells at very high frequencies across the genome. In contrast, gene trapping introduces a “reporter gene” within the coding sequence of a gene, thereby mutating the gene and simultaneously inserting a tag enabling visualization of gene expression and rapid determination of sequence information. Additional mutagenic techniques the CMHD is developing include targeted chromosomal rearrangements, novel mutagenic strategies using embryonic stem cells, and modifier screens.



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